Tuesday, 17th of June
08:00-
09:00
Registration
09:00-
09:20
Opening
09:20-
10:00
Imaging the Molecular Processes of Cell Division Across Scales Jan Ellenberg
10:00-
10:25
Organoid human cancer immunity model in motion Anne Rios
10:25-
10:55
Coffee break
10:55-
11:20
Zooming in and out of cellular ultrastructure with fast 3D electron microscopy Jacob Hoogenboom
11:20-
11:35
High-throughput single molecule microscopy with adaptable spatial resolution using exchangeable oligonucleotide labels Ran Huo
11:35-
11:50
Enabling autonomous, high-throughput single-molecule microscopy Koen Martens
11:50-
12:30
Scaling Life: How Single Cells Orchestrate Tissue-Level Coordination Prisca Liberali
12:30-
13:30
Lunch
13:30-
15:30
Poster session I P
  • P / 1
    Mech(n)anobiology of myeloid cells in 2D and 3D: from podosome nanoscale imaging to T cell immune suppression A. Cambi1 (1Department of Medical BioSciences, Radboud university medical center, Nijmegen, The Netherlands)
  • P / 2
    The role of fibrous corona expansion in spindle formation and microtubule-kinetochore interactions in mammalian oocyte meiosis C. Baggio1,2, A. Larreategui Aparicio, M. De Ruijter-Villani, G.J.P.L. Kops (1Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NL; 2Hubrecht institute, KNAW, Utrecht, NL)
  • P / 3
    Understanding the roles of mitotic spindle orientation in epithelial morphogenesis and tumorigenesis A. Corsini1, T. van Ravesteyn, E. van Kwawegen, G.J.P.L. Kops (1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), and University Medical Center Utrecht, Utrecht, the Netherlands.)
  • P / 4
    Stretching the Limits – Design and Synthesis of a Trivalent Tool for Expansion Microscopy F.C.W.J. Turlings1,2, A.K. Serweta, Z. Wijfjes, A. Arning, F.J. van Dalen, L. Kapitein, M. Verdoes (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands; 2Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands)
  • P / 5
    Exploring cell interactions in the tumor microenvironment C.Y. Liao, E. Neubert, E.H.J. Danen1 (1LACDR, Leiden University)
  • P / 6
    Fluorescence-targeted Lamella Milling with a Coincident Fluorescence, Electron, and Ion Beam Microscope E.G. Perton1,2, D.B. Boltje, C. Taisne, A.J. Jakobi, J.P. Hoogenboom (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands; 2Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands)
  • P / 7
    Label-free 4D-Imaging of Pathophysiological Cell and Tissue Changes in Precision-cut Lung Slices L.A.J. Soeterbroek1,2, N.E. Duits, Y. Ma, M. van Dorp, C. Dickhoff, J.T. Annema, J.W. Duitman, M.L. Groot (1Department of Pulmonary Medicine, Amsterdam UMC, Amsterdam, The Netherlands; 2Department of Experimental Immunology, Amsterdam UMC, Amsterdam, The Netherlands)
  • P / 8
    High-content morphological profiling by Cell Painting in 3D spheroids. C. Ringers1,2,3, D. Holmberg, A. Flobak, P. Georgieva, M. Jarvius, M. Johansson, A. Larsson, B. Seashore-Ludlow, T. Visnes, J. Carreras Puigvert, O. Spjuth (1Department of Pharmaceutical Biosciences and Science for Life Laboratory, Uppsala University, Uppsala, Sweden; 2Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 7034 Trondheim, Norway)
  • P / 9
    Intravital microscopy to track magnesium in the living kidney P.A. Leermakers1, G. Gyarmati, J. Peti-Peterdi, G.J. Bakker, V.I. Shchepetkina, D. Buccella, J.G.J. Hoenderop, J.H.F. de Baaij (1Department of Medical BioSciences, Radboud university medical center, Nijmegen, The Netherlands)
  • P / 10
    Characterization of the GPCR dependencies of TNBC cells using phenotypic screening X. Wang1, L. Ramsaran, B. van de Water, L.H. Heitman, S.E. Le Dévédec (1Department of Cell System and Drug Safety, LACDR, Leiden University, the Netherlands)
  • P / 11
    Examining epithelial alterations in apicobasal polarity during tissue repair using intestinal organoids S.L. Bosman1, D. Kokkinidi, A. Bachsevanoglou Dimitriadou, M.M. Maurice (1Oncode Institute and Department of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, Netherlands)
  • P / 12
    Deciphering the roles of apicobasal polarity in intestinal organoid development D. Kokkinidi1, S.L. Bosman, A. Bachsevanoglou Dimitriadou, M.M. Maurice (1Oncode Institute and Department of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, Netherlands)
  • P / 13
    Microvilli formation in T cells is dependent on tetraspanin CD53  A.N. Kenyon1, A.B. Arp, A.K. Serweta, L.C. Kapitein, S.J. van Deventer, E. Jenkins, M. ter Beest, A.B. van Spriel (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 14
    BIOMERO: A scalable and extensible image analysis framework T.T. Luik1, R. Rosas-Bertolini, E.A.J. Reits, R.A. Hoebe, P.M. Krawczyk (1Amsterdam UMC, Department of Medical Biology, Amsterdam, the Netherlands)
  • P / 15
    Towards the quantitative measurement and control of cAMP and Ca2+ signaling in blood vessels S. Palacios Martinez1,2, J.D. van Buul, T.W.J. Gadella, J. Goedhart (1Molecular Cytology section, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam; 2Medical Biochemistry, Amsterdam UMC)
  • P / 16
    Uncovering human kinetochore heterogeneity and its impact on faithful chromosomal segregation V.T. Victor Tiroille1, G.J.P.L.K. Geert Kops (1Kops Group, Hubrecht Institute, Utrecht, Netherlands)
  • P / 17
    Smart Microscopy: Automated And Efficient Imaging For Life Sciences A. Rates1, J.B. Passmore, M.T.P. van Laarhoven, V.J.W. Hellebrekers, D.M.A. Rietmole, C.S. Smith, I. Smal, L.C. Kapitein (1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands)
  • P / 18
    Adapting Label-Free Higher Harmonic Generation Microscopy to Visualize Cell Dynamics in 3D Human Lung Tissue Culture Y. Ma1, N.B. Lubbersen, S. Spies, A. Dijkhuis, M. van Dorp, C. Dickhoff, J.W. Duitman, M.L. Groot (1LaserLab, Department of Physics, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands)
  • P / 19
    Using computational agent-based models to study the impact of spatial organization on tumor progression and therapy responses B. Wang1,2, F. Eduati (1Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands; 2Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands)
  • P / 20
    Whole-cell 3D DNA-PAINT imaging with water-immersion and field-dependent aberration correction to characterize the molecular organization of the nuclear lamina B.C. Peters1, M.N.F. Hensgens, L.J.E. Andriessen, H.J. Geertsema (1Dept. of Imaging Physics, TU Delft, Delft, Netherlands)
  • P / 21
    Cryo-4Pi single-molecule localization microscopy Q. Li1, M. Wu, Q. Wang, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 22
    Towards a quantitative understanding of the DNA damage response through image-based dynamical modeling M.M. . Heldring, E.J. Burgers, J. Su, L.S. Wijaya, M. Niemeijer, H. Yang, T. Lakhal, S.E. Le Dévédec, B. van de Water, J.B. Beltman1 (1Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research - Leiden University, Leiden, The Netherlands)
  • P / 23
    FAIR Bioimaging Research Infrastructure: The NL-Bioimaging Town Metro Map K.J. Wolstencroft1 (1NL-Bioimaging Consortium; Data Management and Analysis Team)
  • P / 24
    From electron microscope to a potential new treatment for mycobacterial infections S. van der Niet1, K.D. Green, I.M. Schimmel, J. de Bakker, B. Lodder, S. Garneau-Tsodikova, N.N. van der Wel (1Electron Microscopy Center Amsterdam, Department of Medical Biology, Amsterdam UMC, Amsterdam, The Netherlands)
  • P / 25
    Image quality optimization in multi-beam scanning transmission electron microscopy of serial sections S. Karaçoban1, M. Kormacheva, J. Raude, A.A. Wanner, J. Hoogenboom (1Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 26
    Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view I.E.A.C. Droste1, E. Schuitema, S.A. Khan, M.N.F. Hensgens, S. Heldens, C.S. Smith, B. van Werkhoven, H.J. Geertsema, K.A. Lidke, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 27
    Versatile platform for intravital and live cell 3- and 4-photon microscopy G.J. Bakker1, P. Leermakers, N. Wen, L. Teufel, E. Wagena, P. Friedl (1Dept. Medical Biosciences Radboud UMC, Nijmegen, The Netherlands)
  • P / 28
    Patient-Derived Organoids: Exploring Disease Mechanisms Through Functional and Phenotypic Imaging M.S. Doorn1, J. Frias Aldeguer, A. Merenda, R.M. Overmeer, R.G.J. Vries, S.F. Boj (1HUB Organoids B.V., Yalelaan 62, 3584CM, Utrecht, the Netherlands)
  • P / 29
    A HUMAN IPSC-DERIVED MAMMARY GLAND ORGANOID MODEL THAT PHENOTYPICALLY AND FUNCTIONALLY RECAPITULATES HUMAN BIOLOGY R.R. Nair1, R.J.P. Bouwman, B. van de Water, C.L.G.J. Scheele (1Leiden Academic Centre for Drug Research, Universiteit Leiden, The Netherlands)
  • P / 30
    Nanographene: A Promising Fluorophore Facilitating Cryogenic Single-Molecule Localization Microscopy Y. Wang1, Q. Yang, X. Liu, Q. Li, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 31
    Super-resolved FRET in live cells by SOFI H. Valenta1, M. Dalla Vecchia, W. Vandenberg, P. Dedecker (1Department of Chemistry, KU Leuven, Leuven, Belgium)
  • P / 32
    Innovative modelling of the human microcirculation: using RhoB kinetics for in silico modeling of proteostasis J. Kiers1,2, M.C. Overboom, A. Acquaviva, V.S. Muniraj, A.G. Hoekstra, P.L. Hordijk (11Amsterdam UMC location Vrije Universiteit Amsterdam, Department of Physiology, De Boelelaan 1117, Amsterdam, The Netherlands; 22Amsterdam Cardiovascular Sciences, Microcirculation, Atherosclerosis & Aortic disease, Amsterdam, The Netherlands)
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  • P / 34
    Integrating Reproducible Image Analysis pipelines with OMERO M.W. Paul1,2, S.E. Le Dévédec, L.M. Voortman (1Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands; 2Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 35
    Basal body blueprint: Expansion microscopy of microtubules in multiciliated cells E.J. van Grinsven1, E.A. Katrukha, K.B. van den Anker, J.M. Beekman, L.C. Kapitein, A. Akhmanova (1Cell Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 Utrecht, the Netherlands)
  • P / 36
    The TriScan: fast and sensitive 3D fluorescence imaging using a simple optical design R. Van den Eynde1, J. Verheyen, P. Miclea, J. Lazar, W. Vandenberg, P. Dedecker (1Department of Chemistry, KU Leuven, Belgium)
  • P / 37
    Non-invasive live-cell STED imaging S.J. Dijt1, T.C.Q. Burgers, F.N. Mol, J. Matthias, T.M. Maunz, A. Fischer, R. Vlijm (1Molecular Biophysics, Zernike Institute for Advanced Materials, Rijksuniversiteit Groningen, Groningen, the Netherlands,)
  • P / 38
    StableSOLS: A stable and robust implementation of single-objective light sheet microscope. S.A. Sergey Abakumov1, R.V.D.E. Robin Van Den Eynde, W.V. Wim Vandenberg, P.D. Peter Dedecker (1Laboratory for Nanobiology, KULeuven, Leuven, 3000, Belgium)
  • P / 39
    Validating the veracity of 3D Segmentations M.B. Smith1, E. Mulder, Y. Dang, S. Sebestyen, J. Beekman, S. Van Beuningen (1AI lab for Living Technologies & Department for Cellular Disease Models – University Medical Centre Utrecht, The Netherlands)
  • P / 40
    A simple buffer to enhance the performance of the green-emitting fluorophores A. Bourges1, R. Van den Eynde, V. Glembockyte, W. Vandenberg (1Department of Chemistry, KU Leuven, Belgium)
  • P / 41
    Live-cell imaging of extracellular vesicle fusion with target cells in space and time J. van den Ende1, K.A.Y. Defourny, H.H. Rabouw, M.E. Tanenbaum, R.W. Wubbolts, E.N.M. Nolte-'t Hoen (1Division of Infection Biology, Utrecht University)
  • P / 42
    Data-driven parameter-free optimal deconvolution J.D. Manton1, J. Becker, C.T. Russell, A.G. York (1MRC Laboratory of Molecular Biology, Cambridge, UK)
  • P / 43
    Direct-view oblique plane microscopy J.R. Lamb1, M.C. Mestre, M Lancaster, J.D. Manton (1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, United Kingdom, CB2 0QH)
  • P / 44
    A multiscale pipeline for 3D image analysis of intact cerebral organoids B.J.B. Voesenek1, E. Daoutsali, J.W. Rutten, S.A.J. Lesnik-Oberstein, W.M.C. van Roon-Mom, R.A.M. Buijsen (1Department of Human Genetics, Leiden University Medical Center, Leiden, Netherlands)
  • P / 45
    A multi-modal microscopy approach in support of a cell-based muscle therapy M. Willemse1, R.H.L. Raaijmakers, E. Wagena, E. van Beusekom, C.R.M. Ausems, P. Friedl, H. van Bokhoven, D.G. Wansink (1Department of Medical BioSciences, Radboudumc, Nijmegen, 6525 GA, The Netherlands)
  • P / 46
    Use of AI based label free predictions for primary airway epithelial cell assays B.M. Bosch1,2, D. Hoenselaar, L.A. den Hertog - Oosterhof, G.D.. Amatngalim, J.M. Beekman, S.F.B. van Beuningen (1AI lab for living technologies; 2Department for Cellular Disease Models)
  • P / 47
    RNA splicing reprogramming to combat triple-negative breast cancer N. Liu1, J.J.S. van de Velde, S. Ramdjielal, E. Koedoot, N.K. van Overbeek, D. Batenburg, A.C.O. Vertegaal, B. van de Water, S.E. Le Dévédec (1Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands)
  • P / 48
    Cytoskeletal forces and their impact on nuclear lamina organisation M.N.F. Hensgens1, B. Peters, W. van der Voort, A. Mhaskar, H. Geertsema (1Imaging Physics, Delft University of Technology)
  • P / 49
    Impact of lamin-A/C on cancer progression in vivo K.A. Wolf1, M. Svoren, E. Wagena, M. te LIndert, M. Vullings, L. Marcus, P. Friedl (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 50
    Collective behaviour modulation of invading melanoma cells during therapy response. S.A. den Daas1, E. Weinholtz, L. Nasr, M. Vullings, S. Syga, A. Deutsch, P. Friedl (1Department of Medical BioSciences, RadboudUMC, Nijmegen 6525GA, the Netherlands)
  • P / 51
    3D Super-Resolution Imaging of Homologous Chromosome Pairing in Meiosis A.N. Mhaskar1,2, M.N.F. Hensgens, B.C. Peters, E. Sleddens-Linkels, J.A. Slotman, H.J. Geertsema, W.M. Baarends (1Departments of Developmental Biology, Erasmus University Medical Centre, Rotterdam, The Netherlands; 2Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 52
     Imaging Antigen dynamics in Follicular Dendritic Cells L.T. Krimpenfort1 (1Utrecht University, Dept. of Pharmaceutical Science, Div. of Chemical Biology and Drug Discovery)
  • P / 53
    Vascular heterogeneity determines leukocyte transendothelial migration efficiency M.E. Tebbens1, S.K.H. Morsing, J.J.G. Arts, J.D. van Buul (1Vascular Cell Biology Lab at Dept. Medical Biochemistry at the Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands)
  • P / 54
    From Light to Insight: A FLIM Multiplexing and AI-Driven Analysis Pipeline for 3D Organoids allows identification of immunotherapy targets E. Bokobza1,2, R. Van Ineveld, M. Barrera-Roman, M. Molleson, L. Hoestland, A. Zeeman, M. Van der Kruit, A. Rios (1Prinses Maxima Centrum, Utrecht, The Netherlands; 2Oncode Institute, Utrecht, The Netherlands)
  • P / 55
    Human iPSC-derived kidney organoids for renal toxicity safety assessment L.S. Wijaya, M. Desain, M. Hochane, L. van den Berk, K. Snijder, C.W. van den Berg, T.J. Rabelink, B. van de Water, S.E. Le Dévédec1 (1Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 56
    Enhancing AI-Readiness of Bioimaging Data with Content-Based Identifiers (BIO-CODES) T. Pan, M.W. Paul, J. Moore, L.M. Voortman, J.J. Willemse, K.J. Wolstencroft, S. Posth, S.E. Le Dévédec7 (7NL-Bioimaging, Leiden Cell Observatory, Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 57
    Label-free microscopic analysis of exocrine-endocrine dynamics in living zebrafish pancreas N. Faraj1, E.M.F. de Lange, B.N.G. Giepmans (1Department of Biomedical Sciences, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.)
  • P / 58
    BigTrace: a plugin to analyze 3D filaments in time and space E.A. Katrukha1, A. Akhmanova, L.C. Kapitein (1Cell Biology Department, Utrecht University)
  • P / 59
    Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body[1] E.S. Berrevoets1, L.F. Kessler, A. Balakrishnan, M. Müller-McNicoll, B. Rieger, S. Stallinga, M. Heilemann (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 60
    Smart microscopy to monitor responses of CTL function and membrane repair in response to cytoskeletal inhibition therapy  A.A. Berends1, D. Smits, T. Li, A. Rates Soriano, V.J.W. Hellebrekers, L.U. Teufel, E. Wagena, I.V. Smal, L.C. Kapitein, P.H.A. Friedl (1Dept. of Medical BioSciences, Radboud University Medical Centre, Nijmegen, The Netherlands)
  • P / 61
    Environmental cues cause morphological changes in chemosensory cilia G.H. Haasnoot1, C.W. Bruggeman, E.J.G. Peterman (1Vrije Universiteit Amsterdam)
  • P / 62
    Combined light-sheet and X-ray microscopy for multi-scale imaging of organisms across size ranges N. Fuchs1, K. Kairišs, J. Mayer, V. Weinhardt (1Centre for Organismal Studies Heidelberg, Heidelberg University, Germany)
  • P / 63
    A novel single objective oblique-plane microscope for live imaging of organoids F. Berto1, D. Kromm, C. Smith (1Delft University Of Technology, Delft Center for Systems and Control, Mekelweg 2, 2628 CD, Delft, Netherlands)
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  • P / 65
    Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology J.B. Passmore1,2, A. Rates, J. Schroeder, M.T.P. van Laarhoven, V.J.W. Hellebrekers, H.G. van Hoef, A.J.M. Geurts, W. van Straaten, W. Nijenhuis, F. Berger, C.S. Smith, I. Smal, L.C. Kapitein (1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; 2Centre for Living Technologies, Alliance TU/e, WUR, UU, UMC Utrecht, Utrecht, The Netherlands)
  • P / 66
    Characterization and usage of fluorescent tools to study cellular behavior of budding yeast D.B. Botman1, S.K. Kanagasabapathi, D.H.G. de Groot, K.R. Rossum, P.S. Savakis, M.W. Wehrens, J.G. Goedhart, J.H. van Heerden, F.B. Bruggeman, B.T. Teusink (1Systems Biology Lab, Amsterdam Institute of Molecular and Life Sciences (AIMMS). Vrije Universiteit Amsterdam, De Boelelaan 1108, NL-1081HZ Amsterdam, the Netherlands.)
  • P / 67
    Outcome-Driven Microscopy: Spatiotemporal regulation of nucleocytoplasmic transport M.T.P. van Laarhoven1, J.B. Passmore, A. Rates, V.J.W. Hellebrekers, I. Smal, L.C. Kapitein, C. Smith (1Delft Center for Systems & Control, Mechanical Engineering, Delft University of Technology)
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  • P / 69
    Fast and high-throughput Single-Molecule Microscopy by Fluctuation Imaging K. De Zwaan, R. Huo, M. Hensgens, M. Tekpinar, H.L. Wienecke, H. Geertsema, N. Tömen, K.S. Grußmayer1 (1Department of Bionanoscience and Kavli Institute of Nanoscience Delft, TU Delft, Netherlands)
  • P / 70
    Modeling primary ciliary dyskinesia in a native ciliary environment: high-resolution imaging and gene editing of human airway epithelial cells N. Ithakisiou1, P. Cleijpool, B. Yetkin-Arik, E. Katrukha, L.C. Kapitein, J.M. Beekman (1Lab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, UMC Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands)
  • P / 71
    Augmentation in disentangled latent space for one-shot segmentation D.M.A. te Rietmole1, A. Rates, J.B. Passmore, L.C. Kapitein, I. Smal (1Dept of Cell Biology, Utrecht University, Netherlands)
  • P / 72
    Talin1 Adhesion Morphology and Colocalization with Tensin3 Are Largely Unaffected by Polyacrylamide Substrate Stiffness J. Hajduk, P. Twardawa, Z. Rajfur, Z. Baster1,4 (1Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Łojasiewicza 11, 30-348 Cracow, Poland; 4Laboratory for Cell and Tissue Engineering, Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands)
  • P / 73
    Investigating Therapy Resistance Mechanisms in Collective Cancer Cell Migration  L. Nasr1, S. den Daas, M. Zegers, K. van den Dries, P. Friedl (1Department of Medical Biosciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 74
    An Iterative Frequency-based Approach for GelMap Grid Deformation Correction V.J.W. Hellebrekerd1, J.B. Passmore, L.C. Kapitein, I. Small (1)
  • P / 75
    Nanoscale characterization of interphase and mitotic centromere 3D organization using OligoSTORM reveals boundary effects and metaphase inner centromere geometry N.M. Martins1, I. Maceda-Porto, V. Contessoto, E. Dodero-Rojas, T. Hori, I. Farabella, T. Fukagawa, J. Onuchic, M.A. Marti-Renom, T. Wu (1Harvard Medical School, Dept. of Genetics, Blavatnik Institute, Boston MA, USA)
15:30-
16:00
Tea break
16:00-
16:15
Enlightening the Role of Microtubules in Mesenchymal Migration Joyce Meiring
16:15-
16:30
3D Spatial Organization and Dynamics of Dendritic Cell Podosomes on Decellularized Dermal Matrices Koen van den Dries
16:30-
17:10
Imaging the Evolutionary Journey of Centrosomes and Cilia Monica Bettencourt-Dias
17:10-
17:25
Closing words
17:25-
18:25
Drinks
Wednesday, 18th of June
09:00-09:05
Opening
09:15-
09:45
Adding dimension to intravital Imaging Scott Fraser
09:45-
10:00
Towards data-driven segmentation of cellular ultrastructure through hyperspectral electron microscopy Peter Duinkerken
10:00-
10:15
Dissecting the cell type-specific subcellular organization in intestinal organoids using Expansion Microscopy Thijs Makaske
10:15-
10:45
Coffee break
10:45-
12:45
Workshop 1 NL-BioImaging
  • Characterization of the GPCR dependencies of TNBC cells using phenotypic screening X. Wang1, L. Ramsaran, B. van de Water, L.H. Heitman, S.E. Le Dévédec (1Department of Cell System and Drug Safety, LACDR, Leiden University, the Netherlands)
  • Enhancing AI-Readiness of Bioimaging Data with Content-Based Identifiers (BIO-CODES) T. Pan, M.W. Paul, J. Moore, L.M. Voortman, J.J. Willemse, K.J. Wolstencroft, S. Posth, S.E. Le Dévédec7 (7NL-Bioimaging, Leiden Cell Observatory, Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • Label-free 4D-Imaging of Pathophysiological Cell and Tissue Changes in Precision-cut Lung Slices L.A.J. Soeterbroek1,2, N.E. Duits, Y. Ma, M. van Dorp, C. Dickhoff, J.T. Annema, J.W. Duitman, M.L. Groot (1Department of Pulmonary Medicine, Amsterdam UMC, Amsterdam, The Netherlands; 2Department of Experimental Immunology, Amsterdam UMC, Amsterdam, The Netherlands)
  • Live-cell imaging of extracellular vesicle fusion with target cells in space and time J. van den Ende1, K.A.Y. Defourny, H.H. Rabouw, M.E. Tanenbaum, R.W. Wubbolts, E.N.M. Nolte-'t Hoen (1Division of Infection Biology, Utrecht University)
10:45-
12:45
Workshop 2 3D-Nanoscale Imaging
  • A novel single objective oblique-plane microscope for live imaging of organoids F. Berto1, D. Kromm, C. Smith (1Delft University Of Technology, Delft Center for Systems and Control, Mekelweg 2, 2628 CD, Delft, Netherlands)
  • Adapting Label-Free Higher Harmonic Generation Microscopy to Visualize Cell Dynamics in 3D Human Lung Tissue Culture Y. Ma1, N.B. Lubbersen, S. Spies, A. Dijkhuis, M. van Dorp, C. Dickhoff, J.W. Duitman, M.L. Groot (1LaserLab, Department of Physics, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands)
  • Data-driven parameter-free optimal deconvolution J.D. Manton1, J. Becker, C.T. Russell, A.G. York (1MRC Laboratory of Molecular Biology, Cambridge, UK)
  • Fast and high-throughput Single-Molecule Microscopy by Fluctuation Imaging K. De Zwaan, R. Huo, M. Hensgens, M. Tekpinar, H.L. Wienecke, H. Geertsema, N. Tömen, K.S. Grußmayer1 (1Department of Bionanoscience and Kavli Institute of Nanoscience Delft, TU Delft, Netherlands)
  • Non-invasive live-cell STED imaging S.J. Dijt1, T.C.Q. Burgers, F.N. Mol, J. Matthias, T.M. Maunz, A. Fischer, R. Vlijm (1Molecular Biophysics, Zernike Institute for Advanced Materials, Rijksuniversiteit Groningen, Groningen, the Netherlands,)
  • Revealing structural heterogeneity in mitotic chromosomes using optical tweezers and STED microscopy C.P. van der Smagt1, J. Harju, T. Man, A.S. Biebricher, I. Heller, C.P. Broedersz, G.J.L. Wuite, E.J.G. Peterman (1Dept. of Physics and Astronomy, Vrije Universiteit Amsterdam)
  • Super-resolved FRET in live cells by SOFI H. Valenta1, M. Dalla Vecchia, W. Vandenberg, P. Dedecker (1Department of Chemistry, KU Leuven, Leuven, Belgium)
12:45-
13:45
Lunch
13:45-
15:45
Poster session II P
  • P / 1
    Mech(n)anobiology of myeloid cells in 2D and 3D: from podosome nanoscale imaging to T cell immune suppression A. Cambi1 (1Department of Medical BioSciences, Radboud university medical center, Nijmegen, The Netherlands)
  • P / 2
    The role of fibrous corona expansion in spindle formation and microtubule-kinetochore interactions in mammalian oocyte meiosis C. Baggio1,2, A. Larreategui Aparicio, M. De Ruijter-Villani, G.J.P.L. Kops (1Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NL; 2Hubrecht institute, KNAW, Utrecht, NL)
  • P / 3
    Understanding the roles of mitotic spindle orientation in epithelial morphogenesis and tumorigenesis A. Corsini1, T. van Ravesteyn, E. van Kwawegen, G.J.P.L. Kops (1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), and University Medical Center Utrecht, Utrecht, the Netherlands.)
  • P / 4
    Stretching the Limits – Design and Synthesis of a Trivalent Tool for Expansion Microscopy F.C.W.J. Turlings1,2, A.K. Serweta, Z. Wijfjes, A. Arning, F.J. van Dalen, L. Kapitein, M. Verdoes (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands; 2Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands)
  • P / 5
    Exploring cell interactions in the tumor microenvironment C.Y. Liao, E. Neubert, E.H.J. Danen1 (1LACDR, Leiden University)
  • P / 6
    Fluorescence-targeted Lamella Milling with a Coincident Fluorescence, Electron, and Ion Beam Microscope E.G. Perton1,2, D.B. Boltje, C. Taisne, A.J. Jakobi, J.P. Hoogenboom (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands; 2Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands)
  • P / 7
    Label-free 4D-Imaging of Pathophysiological Cell and Tissue Changes in Precision-cut Lung Slices L.A.J. Soeterbroek1,2, N.E. Duits, Y. Ma, M. van Dorp, C. Dickhoff, J.T. Annema, J.W. Duitman, M.L. Groot (1Department of Pulmonary Medicine, Amsterdam UMC, Amsterdam, The Netherlands; 2Department of Experimental Immunology, Amsterdam UMC, Amsterdam, The Netherlands)
  • P / 8
    High-content morphological profiling by Cell Painting in 3D spheroids. C. Ringers1,2,3, D. Holmberg, A. Flobak, P. Georgieva, M. Jarvius, M. Johansson, A. Larsson, B. Seashore-Ludlow, T. Visnes, J. Carreras Puigvert, O. Spjuth (1Department of Pharmaceutical Biosciences and Science for Life Laboratory, Uppsala University, Uppsala, Sweden; 2Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 7034 Trondheim, Norway)
  • P / 9
    Intravital microscopy to track magnesium in the living kidney P.A. Leermakers1, G. Gyarmati, J. Peti-Peterdi, G.J. Bakker, V.I. Shchepetkina, D. Buccella, J.G.J. Hoenderop, J.H.F. de Baaij (1Department of Medical BioSciences, Radboud university medical center, Nijmegen, The Netherlands)
  • P / 10
    Characterization of the GPCR dependencies of TNBC cells using phenotypic screening X. Wang1, L. Ramsaran, B. van de Water, L.H. Heitman, S.E. Le Dévédec (1Department of Cell System and Drug Safety, LACDR, Leiden University, the Netherlands)
  • P / 11
    Examining epithelial alterations in apicobasal polarity during tissue repair using intestinal organoids S.L. Bosman1, D. Kokkinidi, A. Bachsevanoglou Dimitriadou, M.M. Maurice (1Oncode Institute and Department of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, Netherlands)
  • P / 12
    Deciphering the roles of apicobasal polarity in intestinal organoid development D. Kokkinidi1, S.L. Bosman, A. Bachsevanoglou Dimitriadou, M.M. Maurice (1Oncode Institute and Department of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, Netherlands)
  • P / 13
    Microvilli formation in T cells is dependent on tetraspanin CD53  A.N. Kenyon1, A.B. Arp, A.K. Serweta, L.C. Kapitein, S.J. van Deventer, E. Jenkins, M. ter Beest, A.B. van Spriel (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 14
    BIOMERO: A scalable and extensible image analysis framework T.T. Luik1, R. Rosas-Bertolini, E.A.J. Reits, R.A. Hoebe, P.M. Krawczyk (1Amsterdam UMC, Department of Medical Biology, Amsterdam, the Netherlands)
  • P / 15
    Towards the quantitative measurement and control of cAMP and Ca2+ signaling in blood vessels S. Palacios Martinez1,2, J.D. van Buul, T.W.J. Gadella, J. Goedhart (1Molecular Cytology section, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam; 2Medical Biochemistry, Amsterdam UMC)
  • P / 16
    Uncovering human kinetochore heterogeneity and its impact on faithful chromosomal segregation V.T. Victor Tiroille1, G.J.P.L.K. Geert Kops (1Kops Group, Hubrecht Institute, Utrecht, Netherlands)
  • P / 17
    Smart Microscopy: Automated And Efficient Imaging For Life Sciences A. Rates1, J.B. Passmore, M.T.P. van Laarhoven, V.J.W. Hellebrekers, D.M.A. Rietmole, C.S. Smith, I. Smal, L.C. Kapitein (1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands)
  • P / 18
    Adapting Label-Free Higher Harmonic Generation Microscopy to Visualize Cell Dynamics in 3D Human Lung Tissue Culture Y. Ma1, N.B. Lubbersen, S. Spies, A. Dijkhuis, M. van Dorp, C. Dickhoff, J.W. Duitman, M.L. Groot (1LaserLab, Department of Physics, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands)
  • P / 19
    Using computational agent-based models to study the impact of spatial organization on tumor progression and therapy responses B. Wang1,2, F. Eduati (1Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands; 2Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands)
  • P / 20
    Whole-cell 3D DNA-PAINT imaging with water-immersion and field-dependent aberration correction to characterize the molecular organization of the nuclear lamina B.C. Peters1, M.N.F. Hensgens, L.J.E. Andriessen, H.J. Geertsema (1Dept. of Imaging Physics, TU Delft, Delft, Netherlands)
  • P / 21
    Cryo-4Pi single-molecule localization microscopy Q. Li1, M. Wu, Q. Wang, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 22
    Towards a quantitative understanding of the DNA damage response through image-based dynamical modeling M.M. . Heldring, E.J. Burgers, J. Su, L.S. Wijaya, M. Niemeijer, H. Yang, T. Lakhal, S.E. Le Dévédec, B. van de Water, J.B. Beltman1 (1Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research - Leiden University, Leiden, The Netherlands)
  • P / 23
    FAIR Bioimaging Research Infrastructure: The NL-Bioimaging Town Metro Map K.J. Wolstencroft1 (1NL-Bioimaging Consortium; Data Management and Analysis Team)
  • P / 24
    From electron microscope to a potential new treatment for mycobacterial infections S. van der Niet1, K.D. Green, I.M. Schimmel, J. de Bakker, B. Lodder, S. Garneau-Tsodikova, N.N. van der Wel (1Electron Microscopy Center Amsterdam, Department of Medical Biology, Amsterdam UMC, Amsterdam, The Netherlands)
  • P / 25
    Image quality optimization in multi-beam scanning transmission electron microscopy of serial sections S. Karaçoban1, M. Kormacheva, J. Raude, A.A. Wanner, J. Hoogenboom (1Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 26
    Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view I.E.A.C. Droste1, E. Schuitema, S.A. Khan, M.N.F. Hensgens, S. Heldens, C.S. Smith, B. van Werkhoven, H.J. Geertsema, K.A. Lidke, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 27
    Versatile platform for intravital and live cell 3- and 4-photon microscopy G.J. Bakker1, P. Leermakers, N. Wen, L. Teufel, E. Wagena, P. Friedl (1Dept. Medical Biosciences Radboud UMC, Nijmegen, The Netherlands)
  • P / 28
    Patient-Derived Organoids: Exploring Disease Mechanisms Through Functional and Phenotypic Imaging M.S. Doorn1, J. Frias Aldeguer, A. Merenda, R.M. Overmeer, R.G.J. Vries, S.F. Boj (1HUB Organoids B.V., Yalelaan 62, 3584CM, Utrecht, the Netherlands)
  • P / 29
    A HUMAN IPSC-DERIVED MAMMARY GLAND ORGANOID MODEL THAT PHENOTYPICALLY AND FUNCTIONALLY RECAPITULATES HUMAN BIOLOGY R.R. Nair1, R.J.P. Bouwman, B. van de Water, C.L.G.J. Scheele (1Leiden Academic Centre for Drug Research, Universiteit Leiden, The Netherlands)
  • P / 30
    Nanographene: A Promising Fluorophore Facilitating Cryogenic Single-Molecule Localization Microscopy Y. Wang1, Q. Yang, X. Liu, Q. Li, S. Stallinga, B. Rieger (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 31
    Super-resolved FRET in live cells by SOFI H. Valenta1, M. Dalla Vecchia, W. Vandenberg, P. Dedecker (1Department of Chemistry, KU Leuven, Leuven, Belgium)
  • P / 32
    Innovative modelling of the human microcirculation: using RhoB kinetics for in silico modeling of proteostasis J. Kiers1,2, M.C. Overboom, A. Acquaviva, V.S. Muniraj, A.G. Hoekstra, P.L. Hordijk (11Amsterdam UMC location Vrije Universiteit Amsterdam, Department of Physiology, De Boelelaan 1117, Amsterdam, The Netherlands; 22Amsterdam Cardiovascular Sciences, Microcirculation, Atherosclerosis & Aortic disease, Amsterdam, The Netherlands)
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  • P / 34
    Integrating Reproducible Image Analysis pipelines with OMERO M.W. Paul1,2, S.E. Le Dévédec, L.M. Voortman (1Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands; 2Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 35
    Basal body blueprint: Expansion microscopy of microtubules in multiciliated cells E.J. van Grinsven1, E.A. Katrukha, K.B. van den Anker, J.M. Beekman, L.C. Kapitein, A. Akhmanova (1Cell Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 Utrecht, the Netherlands)
  • P / 36
    The TriScan: fast and sensitive 3D fluorescence imaging using a simple optical design R. Van den Eynde1, J. Verheyen, P. Miclea, J. Lazar, W. Vandenberg, P. Dedecker (1Department of Chemistry, KU Leuven, Belgium)
  • P / 37
    Non-invasive live-cell STED imaging S.J. Dijt1, T.C.Q. Burgers, F.N. Mol, J. Matthias, T.M. Maunz, A. Fischer, R. Vlijm (1Molecular Biophysics, Zernike Institute for Advanced Materials, Rijksuniversiteit Groningen, Groningen, the Netherlands,)
  • P / 38
    StableSOLS: A stable and robust implementation of single-objective light sheet microscope. S.A. Sergey Abakumov1, R.V.D.E. Robin Van Den Eynde, W.V. Wim Vandenberg, P.D. Peter Dedecker (1Laboratory for Nanobiology, KULeuven, Leuven, 3000, Belgium)
  • P / 39
    Validating the veracity of 3D Segmentations M.B. Smith1, E. Mulder, Y. Dang, S. Sebestyen, J. Beekman, S. Van Beuningen (1AI lab for Living Technologies & Department for Cellular Disease Models – University Medical Centre Utrecht, The Netherlands)
  • P / 40
    A simple buffer to enhance the performance of the green-emitting fluorophores A. Bourges1, R. Van den Eynde, V. Glembockyte, W. Vandenberg (1Department of Chemistry, KU Leuven, Belgium)
  • P / 41
    Live-cell imaging of extracellular vesicle fusion with target cells in space and time J. van den Ende1, K.A.Y. Defourny, H.H. Rabouw, M.E. Tanenbaum, R.W. Wubbolts, E.N.M. Nolte-'t Hoen (1Division of Infection Biology, Utrecht University)
  • P / 42
    Data-driven parameter-free optimal deconvolution J.D. Manton1, J. Becker, C.T. Russell, A.G. York (1MRC Laboratory of Molecular Biology, Cambridge, UK)
  • P / 43
    Direct-view oblique plane microscopy J.R. Lamb1, M.C. Mestre, M Lancaster, J.D. Manton (1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, United Kingdom, CB2 0QH)
  • P / 44
    A multiscale pipeline for 3D image analysis of intact cerebral organoids B.J.B. Voesenek1, E. Daoutsali, J.W. Rutten, S.A.J. Lesnik-Oberstein, W.M.C. van Roon-Mom, R.A.M. Buijsen (1Department of Human Genetics, Leiden University Medical Center, Leiden, Netherlands)
  • P / 45
    A multi-modal microscopy approach in support of a cell-based muscle therapy M. Willemse1, R.H.L. Raaijmakers, E. Wagena, E. van Beusekom, C.R.M. Ausems, P. Friedl, H. van Bokhoven, D.G. Wansink (1Department of Medical BioSciences, Radboudumc, Nijmegen, 6525 GA, The Netherlands)
  • P / 46
    Use of AI based label free predictions for primary airway epithelial cell assays B.M. Bosch1,2, D. Hoenselaar, L.A. den Hertog - Oosterhof, G.D.. Amatngalim, J.M. Beekman, S.F.B. van Beuningen (1AI lab for living technologies; 2Department for Cellular Disease Models)
  • P / 47
    RNA splicing reprogramming to combat triple-negative breast cancer N. Liu1, J.J.S. van de Velde, S. Ramdjielal, E. Koedoot, N.K. van Overbeek, D. Batenburg, A.C.O. Vertegaal, B. van de Water, S.E. Le Dévédec (1Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands)
  • P / 48
    Cytoskeletal forces and their impact on nuclear lamina organisation M.N.F. Hensgens1, B. Peters, W. van der Voort, A. Mhaskar, H. Geertsema (1Imaging Physics, Delft University of Technology)
  • P / 49
    Impact of lamin-A/C on cancer progression in vivo K.A. Wolf1, M. Svoren, E. Wagena, M. te LIndert, M. Vullings, L. Marcus, P. Friedl (1Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 50
    Collective behaviour modulation of invading melanoma cells during therapy response. S.A. den Daas1, E. Weinholtz, L. Nasr, M. Vullings, S. Syga, A. Deutsch, P. Friedl (1Department of Medical BioSciences, RadboudUMC, Nijmegen 6525GA, the Netherlands)
  • P / 51
    3D Super-Resolution Imaging of Homologous Chromosome Pairing in Meiosis A.N. Mhaskar1,2, M.N.F. Hensgens, B.C. Peters, E. Sleddens-Linkels, J.A. Slotman, H.J. Geertsema, W.M. Baarends (1Departments of Developmental Biology, Erasmus University Medical Centre, Rotterdam, The Netherlands; 2Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 52
     Imaging Antigen dynamics in Follicular Dendritic Cells L.T. Krimpenfort1 (1Utrecht University, Dept. of Pharmaceutical Science, Div. of Chemical Biology and Drug Discovery)
  • P / 53
    Vascular heterogeneity determines leukocyte transendothelial migration efficiency M.E. Tebbens1, S.K.H. Morsing, J.J.G. Arts, J.D. van Buul (1Vascular Cell Biology Lab at Dept. Medical Biochemistry at the Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands)
  • P / 54
    From Light to Insight: A FLIM Multiplexing and AI-Driven Analysis Pipeline for 3D Organoids allows identification of immunotherapy targets E. Bokobza1,2, R. Van Ineveld, M. Barrera-Roman, M. Molleson, L. Hoestland, A. Zeeman, M. Van der Kruit, A. Rios (1Prinses Maxima Centrum, Utrecht, The Netherlands; 2Oncode Institute, Utrecht, The Netherlands)
  • P / 55
    Human iPSC-derived kidney organoids for renal toxicity safety assessment L.S. Wijaya, M. Desain, M. Hochane, L. van den Berk, K. Snijder, C.W. van den Berg, T.J. Rabelink, B. van de Water, S.E. Le Dévédec1 (1Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 56
    Enhancing AI-Readiness of Bioimaging Data with Content-Based Identifiers (BIO-CODES) T. Pan, M.W. Paul, J. Moore, L.M. Voortman, J.J. Willemse, K.J. Wolstencroft, S. Posth, S.E. Le Dévédec7 (7NL-Bioimaging, Leiden Cell Observatory, Division of Cell Systems and Drug Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands)
  • P / 57
    Label-free microscopic analysis of exocrine-endocrine dynamics in living zebrafish pancreas N. Faraj1, E.M.F. de Lange, B.N.G. Giepmans (1Department of Biomedical Sciences, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.)
  • P / 58
    BigTrace: a plugin to analyze 3D filaments in time and space E.A. Katrukha1, A. Akhmanova, L.C. Kapitein (1Cell Biology Department, Utrecht University)
  • P / 59
    Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body[1] E.S. Berrevoets1, L.F. Kessler, A. Balakrishnan, M. Müller-McNicoll, B. Rieger, S. Stallinga, M. Heilemann (1Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands)
  • P / 60
    Smart microscopy to monitor responses of CTL function and membrane repair in response to cytoskeletal inhibition therapy  A.A. Berends1, D. Smits, T. Li, A. Rates Soriano, V.J.W. Hellebrekers, L.U. Teufel, E. Wagena, I.V. Smal, L.C. Kapitein, P.H.A. Friedl (1Dept. of Medical BioSciences, Radboud University Medical Centre, Nijmegen, The Netherlands)
  • P / 61
    Environmental cues cause morphological changes in chemosensory cilia G.H. Haasnoot1, C.W. Bruggeman, E.J.G. Peterman (1Vrije Universiteit Amsterdam)
  • P / 62
    Combined light-sheet and X-ray microscopy for multi-scale imaging of organisms across size ranges N. Fuchs1, K. Kairišs, J. Mayer, V. Weinhardt (1Centre for Organismal Studies Heidelberg, Heidelberg University, Germany)
  • P / 63
    A novel single objective oblique-plane microscope for live imaging of organoids F. Berto1, D. Kromm, C. Smith (1Delft University Of Technology, Delft Center for Systems and Control, Mekelweg 2, 2628 CD, Delft, Netherlands)
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  • P / 65
    Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology J.B. Passmore1,2, A. Rates, J. Schroeder, M.T.P. van Laarhoven, V.J.W. Hellebrekers, H.G. van Hoef, A.J.M. Geurts, W. van Straaten, W. Nijenhuis, F. Berger, C.S. Smith, I. Smal, L.C. Kapitein (1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands; 2Centre for Living Technologies, Alliance TU/e, WUR, UU, UMC Utrecht, Utrecht, The Netherlands)
  • P / 66
    Characterization and usage of fluorescent tools to study cellular behavior of budding yeast D.B. Botman1, S.K. Kanagasabapathi, D.H.G. de Groot, K.R. Rossum, P.S. Savakis, M.W. Wehrens, J.G. Goedhart, J.H. van Heerden, F.B. Bruggeman, B.T. Teusink (1Systems Biology Lab, Amsterdam Institute of Molecular and Life Sciences (AIMMS). Vrije Universiteit Amsterdam, De Boelelaan 1108, NL-1081HZ Amsterdam, the Netherlands.)
  • P / 67
    Outcome-Driven Microscopy: Spatiotemporal regulation of nucleocytoplasmic transport M.T.P. van Laarhoven1, J.B. Passmore, A. Rates, V.J.W. Hellebrekers, I. Smal, L.C. Kapitein, C. Smith (1Delft Center for Systems & Control, Mechanical Engineering, Delft University of Technology)
  •    This item has been removed from the program.
  • P / 69
    Fast and high-throughput Single-Molecule Microscopy by Fluctuation Imaging K. De Zwaan, R. Huo, M. Hensgens, M. Tekpinar, H.L. Wienecke, H. Geertsema, N. Tömen, K.S. Grußmayer1 (1Department of Bionanoscience and Kavli Institute of Nanoscience Delft, TU Delft, Netherlands)
  • P / 70
    Modeling primary ciliary dyskinesia in a native ciliary environment: high-resolution imaging and gene editing of human airway epithelial cells N. Ithakisiou1, P. Cleijpool, B. Yetkin-Arik, E. Katrukha, L.C. Kapitein, J.M. Beekman (1Lab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, UMC Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands)
  • P / 71
    Augmentation in disentangled latent space for one-shot segmentation D.M.A. te Rietmole1, A. Rates, J.B. Passmore, L.C. Kapitein, I. Smal (1Dept of Cell Biology, Utrecht University, Netherlands)
  • P / 72
    Talin1 Adhesion Morphology and Colocalization with Tensin3 Are Largely Unaffected by Polyacrylamide Substrate Stiffness J. Hajduk, P. Twardawa, Z. Rajfur, Z. Baster1,4 (1Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Łojasiewicza 11, 30-348 Cracow, Poland; 4Laboratory for Cell and Tissue Engineering, Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands)
  • P / 73
    Investigating Therapy Resistance Mechanisms in Collective Cancer Cell Migration  L. Nasr1, S. den Daas, M. Zegers, K. van den Dries, P. Friedl (1Department of Medical Biosciences, Radboud University Medical Center, Nijmegen, The Netherlands)
  • P / 74
    An Iterative Frequency-based Approach for GelMap Grid Deformation Correction V.J.W. Hellebrekerd1, J.B. Passmore, L.C. Kapitein, I. Small (1)
  • P / 75
    Nanoscale characterization of interphase and mitotic centromere 3D organization using OligoSTORM reveals boundary effects and metaphase inner centromere geometry N.M. Martins1, I. Maceda-Porto, V. Contessoto, E. Dodero-Rojas, T. Hori, I. Farabella, T. Fukagawa, J. Onuchic, M.A. Marti-Renom, T. Wu (1Harvard Medical School, Dept. of Genetics, Blavatnik Institute, Boston MA, USA)
15:45-
16:15
Tea break
16:15-
16:30
NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding Silke Denis
16:30-
16:45
Multispectral live-cell imaging at uncompromised spatiotemporal resolution Akaash Kumar
16:45-
17:25
Exploring life’s structures across scales through imaging Jennifer Lippincott-Schwartz
17:25-
17:40
Closing words
17:40-
18:40
Drinks